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Recombinant Rat Cytochrome c1 protein (His tag)

Product Specifications

Background

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

Abbreviation

Cytochrome c1

Synonyms

CYC1; Complex III subunit IV; Cytochrome c 1

UniProt

D3ZFQ8

Accession Number

D3ZFQ8

Expression System

E.coli

Tag

N-His

Sequence

Glu 145-Glu 280

Field of Research

Tags & Cell Markers; Signal Transduction; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.9 kDa

Observed Molecular Weight

15 kDa

Species

Rat

Available Sizes

Frequently Asked Questions

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