Recombinant Rat NEFL protein (His tag)

Product Specifications

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

Abbreviation

NEFL

Synonyms

68 kDa neurofilament protein; NF-L; Neurofilament triplet L protein

UniProt

P19527

Accession Number

P19527

Expression System

E.coli

Tag

N-His

Sequence

Phe281-Gly396

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.