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Recombinant Rat NSE protein (His tag)

Product Specifications

Background

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Abbreviation

NSE

Synonyms

2-phospho-D-glycerate hydro-lyase; Enolase 2; NSE; Neural enolase; Neuron-specific enolase

UniProt

P07323

Accession Number

P07323

Expression System

E.coli

Tag

N-His

Sequence

Met1-Leu434

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.0 kDa

Observed Molecular Weight

50 kDa

Species

Rat

Available Sizes

Frequently Asked Questions

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