Recombinant Gekko japonicus PDGFC protein (His tag)

Product Specifications

Background

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene.

Abbreviation

PDGFC

Synonyms

Fallotein; PDGF-C; Platelet derived growth factor C; SCDGF; Secretory growth factor like protein; Spinal cord derived growth factor; VEGF E; VEGF-E

UniProt

A8WCC4

Accession Number

A8WCC4

Expression System

E.coli

Tag

N-His

Sequence

Asp 23-Gly 345

Field of Research

Cancer; Cardiovascular; Signal transduction; Stem cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.