Recombinant Danio rerio Beta-actin/ACTB protein (His Tag)
Product Specifications
Background
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G-and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA
Abbreviation
Beta-actin; ACTB
Synonyms
A26C1A; A26C1B; ACTB; ACTBB; Actin; Actin beta; Actin cytoplasmic 1; Actx; BRWS1; Beta cytoskeletal actin; Beta-actin; E430023M04Rik; MGC128179; N-terminally processed; PS1TP5 binding protein 1; PS1TP5BP1; b actin; cytoplasmic 1
UniProt
Q7ZVF9
Accession Number
Q7ZVF9
Expression System
E.coli
Tag
N-His
Sequence
Met1-Phe375
Applications
Immunogen
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
43.1 kDa
Observed Molecular Weight
42-48 kDa
Species
Danio rerio
Available Sizes
Documents
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