Recombinant E.coli Glycerol kinase Protein (His Tag)
Product Specifications
Background
The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key Enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD) . Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Abbreviation
Glycerol kinase
Synonyms
GK; glpK
UniProt
P0A6F3
Accession Number
P0A6F3
Expression System
E.coli
Tag
N-His & C-His
Sequence
Thr2-Glu502
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
56.1 kDa
Observed Molecular Weight
55-58 kDa
Species
E.coli
Available Sizes
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