Recombinant E.coli Glycerol kinase Protein (His Tag)

Product Specifications

Background

The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key Enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD) . Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Abbreviation

Glycerol kinase

Synonyms

GK; glpK

UniProt

P0A6F3

Accession Number

P0A6F3

Expression System

E.coli

Tag

N-His & C-His

Sequence

Thr2-Glu502

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.