Recombinant Mouse ABL1/c-Abl protein (His tag)

Product Specifications

Background

Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

Abbreviation

ABL1; c-Abl

Synonyms

ABL 1; Abelson tyrosine protein kinase 1; JTK7; Proto-oncogene c-Abl; bcr/abl; p150; v abl

UniProt

P00520

Accession Number

P00520

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Asn 193

Field of Research

Signal Transduction; Cancer; Epigenetics and Nuclear Signaling; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.