Recombinant Mouse MME/CD10/Neprilysin protein (His tag)

Product Specifications

Background

CD10, also known as neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides. CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues. Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation. In addition, CD10+GPR77+ carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance.

Abbreviation

MME; CD10; Neprilysin

Synonyms

Atriopeptidase; CALLA; Enkephalinase; EPN; NEP; Neprilysin; neprilysin-390; neprilysin-411; SFE; Skin fibroblast elastase; Mme; NEP; gp100

UniProt

Q61391

Accession Number

Q61391

Expression System

E.coli

Tag

N-His

Sequence

Met 461-Trp 750

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.