Recombinant Mouse IL1A/IL-1A/IL-1F1 protein (His tag)
Product Specifications
Background
Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.
Abbreviation
IL1A; IL-1A; IL-1F1
Synonyms
Hematopoietin; IL1F; Hematopoietin-1; IL1; IL1-A; IL1F1; IL-1α; Preinterleukin 1 Alpha; Pro-Interleukin-1-Alpha; Il-1a; Interleukin-1 alpha; IL-1 alpha; Il1a
UniProt
P01582
Accession Number
P01582
Expression System
E.coli
Tag
C-His
Sequence
Met 1-Ser 270
Field of Research
Immunology; Metabolism; Neuroscience
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
29.6 kDa
Observed Molecular Weight
35 kDa
Species
Mouse
Available Sizes
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