Recombinant Mouse TNF-α protein (His tag)

Product Specifications

Background

Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

Abbreviation

TNF-α

Synonyms

Cachectin; DIF; Membrane Form; TNF-Alpha; TNF-a; TNF-alpha; TNFSF2; TNFalpha; Tnf; Tnfa; Tnfsf1a; Tnfsf2; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2

UniProt

P06804

Accession Number

P06804

Expression System

E.coli

Tag

N-His

Sequence

Leu 80–Leu 235

Field of Research

Cancer; Cardiovascular; Metabolism Immunology; Signal transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.