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Recombinant Mouse Tn-I protein (His tag)

Product Specifications

Background

Troponin I, also known as TNI and CTF1, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with hereditary cardiomyopathy.

Abbreviation

Tn-I

Synonyms

Troponin I; Tnni3TNNI3Tn-I; Tnni3k

UniProt

P48787

Accession Number

P48787

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Gly 211

Field of Research

Cardiovascular; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.1 kDa

Observed Molecular Weight

32 kDa

Species

Mouse

Available Sizes

Frequently Asked Questions

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