Recombinant Mouse Tn-I protein (His tag)
Product Specifications
Background
Troponin I, also known as TNI and CTF1, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with hereditary cardiomyopathy.
Abbreviation
Tn-I
Synonyms
Troponin I; Tnni3TNNI3Tn-I; Tnni3k
UniProt
P48787
Accession Number
P48787
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Gly 211
Field of Research
Cardiovascular; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
23.1 kDa
Observed Molecular Weight
32 kDa
Species
Mouse
Available Sizes
Frequently Asked Questions
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