Recombinant Mouse CASP1 protein (His tag)
Product Specifications
Background
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits.
Abbreviation
CASP1
Synonyms
CASP-1; CASP1; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC
UniProt
P29452
Accession Number
P29452
Expression System
E.coli
Tag
N-His
Sequence
Asn 120-Asp 296
Field of Research
Cancer; Cell Biology; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/wuuqw9
Product MSDS
https://789.bio/eb/mSlDt9
Calculated Molecular Weight
19.4 kDa
Observed Molecular Weight
21 kDa
Species
Mouse
Available Sizes
Curated Selection
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