Recombinant Mouse INS protein (GST tag)

Product Specifications

Background

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Abbreviation

INS

Synonyms

IDDM; IRDN; Ins-2; Insulin B chain; Proinsulin; ins

UniProt

P01326

Accession Number

P01326

Expression System

E.coli

Tag

N-GST

Sequence

Phe 25-Asn 110

Field of Research

Signal Transduction; Neuroscience; Cardiovascular; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.