Recombinant Human ARF6 protein (His tag)

Product Specifications

Background

GTP-binding protein involved in protein trafficking, regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity) . Contributes to the regulation of dendritic branching and filopodia extension.

Abbreviation

ARF6

Synonyms

ADP-ribosylation factor 6; ARF6

UniProt

P62330

Accession Number

P62330

Expression System

E.coli

Tag

N-His

Sequence

Gly 2-Ser 175

Field of Research

Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.