Recombinant Human ENO1 protein (GST tag)

Product Specifications

Background

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Abbreviation

ENO1

Synonyms

2 phospho D glycerate hydro lyase; Alpha enolase; C myc promoter binding protein; ENO1L1; Enolase 1 (alpha) ; MBP 1; NNE; PPH; Tau crystallin

UniProt

P06733

Accession Number

P06733

Expression System

E.coli

Tag

N-GST

Sequence

Ser 2-Lys 434)

Field of Research

Signal Transduction; Epigenetics and Nuclear Signaling; Cancer; Metabolism; Carbohydrate metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.