Recombinant Human SF3B3 protein (GST tag)
Product Specifications
Background
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Abbreviation
SF3B3
Synonyms
SF3B3; Spliceosome-associated protein 130; Splicing factor 3B subunit 3
UniProt
Q15393
Accession Number
Q15393-3
Expression System
E.coli
Tag
N-GST
Sequence
Glu 250-Phe 399
Field of Research
Epigenetics and Nuclear signaling
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
41.4 kDa
Observed Molecular Weight
42 kDa
Species
Human
Available Sizes
Documents
Frequently Asked Questions
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