Recombinant Human E2F2 protein (His tag)

E2F-2 (viral E2-associated factor 2) is a 50 55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA activating E2F heterodimeric complex with DP-1 or-2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F 2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65 105), a DNA binding domain (aa 107 196), a dimerization segment (aa 197 289), a transactivation region (aa 359 437), and a pRB binding domain (aa 410 427) . There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349 437. Over aa 308 437, human E2F-2 is 72% aa identical to mouse E2F-2.

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.