Recombinant Human hnRNPK protein (GST, His tag)

Product Specifications

Background

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly (C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly (C) single-stranded

Abbreviation

HnRNPK

Synonyms

HNRPK; Heterogeneous nuclear ribonucleoprotein K; Transformation up-regulated nuclear protein (TUNP)

UniProt

P61978

Accession Number

P61978-2

Expression System

E.coli

Tag

N-GST & C-His

Sequence

Met 1-Lys 219

Field of Research

Epigenetics and Nuclear signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.