Recombinant Human LPL protein (His tag)
Product Specifications
Background
LPL (LipoProtein Lipase, also LIPD) is a 53-56 kDa glycoprotein member of the Lipase family, AB Hydrolase superfamily of molecules. It is produced by multiple cell types, including adipocytes, skelelal muscle cells and macrophages. Once secreted, the circulating enzyme ultimately becomes immobilized on the surface of endothelium by binding to cell surface heparan sulfate. Here, it hydrolyzes triglycerides embedded in chylomicrons and VLDLs by homodimerizing and interacting with apoC2.
Abbreviation
LPL
Synonyms
LIPL; LPL LIPD; Lipoprotein lipase (LPL)
UniProt
P06858
Accession Number
P06858
Expression System
E.coli
Tag
N-His
Sequence
Ala 28-Gly 475
Field of Research
Cancer; Cardiovascular; Metabolism; signal Transduction; Stem Cells
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
49.2 kDa
Observed Molecular Weight
55 kDa
Species
Human
Available Sizes
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