Recombinant Human PACT protein (His tag)
Product Specifications
Background
Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.
Abbreviation
PACT
Synonyms
Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PRKRA
UniProt
O75569
Accession Number
O75569
Expression System
E.coli
Tag
N-His & C-His
Sequence
Met 1-Lys 313
Field of Research
Epigenetics and Nuclear signaling
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
34.3 kDa
Observed Molecular Weight
38 kDa
Species
Human
Available Sizes
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