Recombinant Human P-glycoprotein1/ABCB1 protein (His tag)
Product Specifications
Background
Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218, PubMed:35970996) . Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203) . Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218, PubMed:35970996) .
Abbreviation
P-glycoprotein1; ABCB1
Synonyms
ABCB1; ATP-binding cassette sub-family B member 1; ATP-dependent translocase ABCB1; CD243; P-glycoprotein 1; Phospholipid transporter ABCB1
UniProt
P08183
Accession Number
P08183
Expression System
E.coli
Tag
N-His
Sequence
Leu 392-Met 628
Field of Research
Cancer; Neuroscience; signal Transduction; stem Cells; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
26.0 kDa
Observed Molecular Weight
32 kDa
Species
Human
Available Sizes
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