Recombinant Human P-glycoprotein1/ABCB1 protein (His tag)

Product Specifications

Background

Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218, PubMed:35970996) . Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203) . Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218, PubMed:35970996) .

Abbreviation

P-glycoprotein1; ABCB1

Synonyms

ABCB1; ATP-binding cassette sub-family B member 1; ATP-dependent translocase ABCB1; CD243; P-glycoprotein 1; Phospholipid transporter ABCB1

UniProt

P08183

Accession Number

P08183

Expression System

E.coli

Tag

N-His

Sequence

Leu 392-Met 628

Field of Research

Cancer; Neuroscience; signal Transduction; stem Cells; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.