Recombinant Human PP2A-Cα/PPP2CA protein (His tag)

Product Specifications

Background

Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits, a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab) . PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.

Abbreviation

PP2A-Cα; PPP2CA

Synonyms

PP2A-alpha; PPP2CA; Replication protein C (RP-C) ; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

UniProt

P67775

Accession Number

P67775

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Leu 309

Field of Research

Cancer; Signal Transduction; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.