Recombinant Human RPLP1 protein (His, GST tag)

Product Specifications

Background

RPLP1 (ribosomal protein large P1) is part of the 60S ribosomal subunit and functions in regulating elongation translation factors, and its expression is regulated by the estrogen target gene, c-Myc. RPLP1 has two alternatively spliced transcript variants that encode different proteins, with variant 1 coding for a protein of ~12-kDa molecular weight and variant 2 coding for an ~8.4-kDa protein, and the variant 1-transcribed protein appears as the major isoform. RPLP1 is proposed to play a role in diseases associated with cell proliferation and cell survival.

Abbreviation

RPLP1

Synonyms

60S acidic ribosomal protein P1; Large ribosomal subunit protein P1; RPLP1; RRP1

UniProt

P05386

Accession Number

P05386

Expression System

E.coli

Tag

N-GST & C-His

Sequence

Met 1-Asp 114

Field of Research

Epigenetics and Nuclear signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.