Recombinant Human SEC13 protein (His tag)
Product Specifications
Background
Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport.
Abbreviation
SEC13
Synonyms
GATOR complex protein SEC13; Protein SEC13 homolog; SEC13; SEC13-like protein 1; SEC13-related protein
UniProt
P55735
Accession Number
P55735
Expression System
E.coli
Tag
N-His & C-His
Sequence
Met 1-Gln 322
Field of Research
Signal Transduction; Tags and Cell Markers
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
35.3 kDa
Observed Molecular Weight
40 kDa
Species
Human
Available Sizes
Documents
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