Recombinant Human XIAP protein (His tag)

Product Specifications

Background

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and-BIR3 domains.

Abbreviation

XIAP

Synonyms

Baculoviral IAP repeat-containing protein 4; E3 ubiquitin-protein ligase XIAP; IAP-like protein; Inhibitor of apoptosis protein 3; RING-type E3 ubiquitin transferase XIAP; X-linked inhibitor of apoptosis protein; XIAP

UniProt

P98170

Accession Number

P98170

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Ser 497

Field of Research

Cancer; Cell Biology; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.