Recombinant Human GLUT6/SLC2A6 protein (GST, His tag)

Product Specifications

Background

GLUT6 as a functionally uncharacterized transporter that putatively works in inflammatory responses. Inflammatory stimuli increase GLUT6 expression level, although GLUT6-knockout mice exhibit a subtle phenotype to lipopolysaccharide administration. Metabolomics and in vitro analyses show that GLUT6 functions as a glycolysis modulator in inflammatory macrophages. GLUT6 does not mediate glucose uptake and is localized on lysosomal membranes. We conclude that GLUT6 is a lysosomal transporter that is regulated by inflammatory stimuli and modulates inflammatory responses by affecting the metabolic shift in macrophages.

Abbreviation

GLUT6; SLC2A6

Synonyms

GLUT6; Glucose transporter type 6; SLC2A6; Solute carrier family 2; facilitated glucose transporter member 6

UniProt

Q9UGQ3

Accession Number

Q9UGQ3-1

Expression System

E.coli

Tag

N-GST & C-His

Sequence

Asn 216-Thr 289

Field of Research

Metabolism; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.