Recombinant Human PSMA / FOLH1 protein (His tag)

Product Specifications

Background

Glutamate carboxypeptidase 2, also known as FOLH1, PSMA, belongs to the M28B subfamily and the peptidase M28 family. It is highly expressed in prostate epithelium and can be detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level) . PSMA is used as a diagnostic and prognostic indicator of prostate cancer, and as a possible marker for various neurological disorders such as schizophrenia, Alzheimer disease and Huntington disease. It has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. PSMA involves in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, PSMA cleaves Gly-Pro-AMC. PSMA is stable at pH greater than 6.5.

Abbreviation

PSMA; FOLH1

Synonyms

Cell growth-inhibiting gene 27 protein; FGCP; FOLH; FOLH1; Folate hydrolase 1; GCP2; GCPII; Glutamate carboxypeptidase 2; Mgcp; NAALAD1; NAALADase I; NAALAdase; PSM; PSMA; mGCP

UniProt

Q04609

Accession Number

Q04609

Expression System

E.coli

Tag

N-His

Sequence

Tyr 471-Ala 750

Field of Research

Cancer; Metablism; signal Tronsduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.