Recombinant Human PCNA protein (His tag)
Product Specifications
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Abbreviation
PCNA
Synonyms
ATLD2; Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; Polymerase delta accessory protein; Proliferating cell nuclear antigen; cb16
UniProt
P12004
Accession Number
P12004
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Ser 261
Field of Research
Cell Biology; Epigenetics and Nuclear Signaling; Tags & Cell Markers; Cancer; Isotype/Loading Controls
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
28.6 kDa
Observed Molecular Weight
38 kDa
Species
Human
Available Sizes
Documents
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