Recombinant Human PCNA protein (His tag)

Product Specifications

Background

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Abbreviation

PCNA

Synonyms

ATLD2; Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; Polymerase delta accessory protein; Proliferating cell nuclear antigen; cb16

UniProt

P12004

Accession Number

P12004

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ser 261

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Tags & Cell Markers; Cancer; Isotype/Loading Controls

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.