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Recombinant Human ACTA2 protein (His tag)

Product Specifications

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β-and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells.

Abbreviation

ACTA2

Synonyms

AAT6; ACTSA; Actin aortic smooth muscle; GIG46; Growth inhibiting gene 46; MYMY5; a actin

UniProt

P62736

Accession Number

P62736

Expression System

E.coli

Tag

N-His

Sequence

Cys 2-Phe 377

Field of Research

Cardiovascular; Signal Transduction; Stem Cell; Developmental Biology ImmunologySignal Transduction; Stem Cell; Cardiovascular; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.3 kDa

Observed Molecular Weight

42 kDa

Species

Human

Available Sizes

Frequently Asked Questions

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