Recombinant Human P21 protein (His tag)

Product Specifications

Background

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Abbreviation

P21

Synonyms

CAP20; CDK-interacting protein 1; CDKI; CDKN1; CDKN1A; CDN1A; CIP1; Cyclin Dependent Kinase Inhibitor 1A; DNA Synthesis Inhibitor; MDA-6; MDA6; Melanoma differentiation-associated protein 6; P21cip1; P21waf; PIC1; SDI1; SLC12A9; WAF1

UniProt

P38936

Accession Number

P38936

Expression System

E.coli

Tag

N-His

Sequence

Gly 40-Pro 164

Field of Research

Signal Transduction; Cancer; Epigenetics and Nuclear Signaling; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.