Recombinant Human TIMP3 protein (His tag)

Product Specifications

Background

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Abbreviation

TIMP3

Synonyms

HSMRK222; K222TA2; MIG 5 protein; Metalloproteinase inhibitor 3; SFD; TIMP 3; TIMP-3; Tissue Inhibitor of Metalloproteinase 3

UniProt

P35625

Accession Number

P35625

Expression System

E.coli

Tag

N-His

Sequence

Cys 24-Asn 144

Field of Research

Signal Transduction; Cardiovascular; Cancer; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.