Recombinant Human NEDD8 protein (His tag)
Product Specifications
Background
Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Abbreviation
NEDD8
Synonyms
NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; Ubiquitin-like protein Nedd8
UniProt
Q15843
Accession Number
Q15843
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Gly 76
Field of Research
Cell biology
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
8.3 kDa
Observed Molecular Weight
12 kDa
Species
Human
Available Sizes
Frequently Asked Questions
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