Recombinant Human VWFCP/ADAMTS13 protein (His tag)

Product Specifications

Background

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.Zinc and calcium ions cooperatively modulate enzyme activity. The cleavage of the pro-domain is not required for protease activity. Dependence on calcium for proteolytic activity is mediated by the high affinity site.

Abbreviation

VWFCP; ADAMTS13

Synonyms

ADAM-TS 13; ADAMTS 13; ATS13; C9orf8; TTP; Von Willebrand factor cleaving protease; vWF CP; vWF-cleaving protease

UniProt

Q76LX8

Accession Number

Q76LX8

Expression System

E.coli

Tag

N-His

Sequence

Thr 1151-Thr 1427

Field of Research

Signal Transduction; Cancer; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.