Recombinant Human bFGF/FGF2 protein (His tag)

Product Specifications

Background

FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.

Abbreviation

BFGF; FGF2

Synonyms

Basic Fibroblast Growth Factor; Bfgf; FGF-2; FGF2; FGFB; Fibroblast Growth Factor 2; HBGF-2; Heparin-Binding Growth Factor 2

UniProt

P09038

Accession Number

P09038

Expression System

E.coli

Tag

N-His

Sequence

Pro 143-Ser 288

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; Stem cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.