Recombinant Human OGA protein (His tag)
Product Specifications
Background
Isoform 1:Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3 :Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Abbreviation
OGA
Synonyms
HEXC; KIAA0679; MEA5; MGEA5; OGA; Protein O-GlcNAcase
UniProt
O60502
Accession Number
O60502
Expression System
E.coli
Tag
N-His
Sequence
Ser 6-Lys 240
Field of Research
Signal Transduction; Cancer; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
25.7 kDa
Observed Molecular Weight
25 kDa
Species
Human
Available Sizes
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