Recombinant Human Ub protein (His tag)
Product Specifications
Background
Polyubiquitin-B (UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.
Abbreviation
Ub
Synonyms
Polyubiquitin-B; UBB; Ubiquitin; Ubiquitin B
UniProt
P0CG47
Accession Number
P0CG47
Expression System
E.coli
Tag
N-His
Sequence
Lys 103-Cys 229
Field of Research
Cell biology; Neuroscience; epigenetics and nuclear signal
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
13.9 kDa
Observed Molecular Weight
15 kDa
Species
Human
Available Sizes
Documents
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