Recombinant Human Ub protein (His tag)

Product Specifications

Background

Polyubiquitin-B (UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.

Abbreviation

Synonyms

Polyubiquitin-B; UBB; Ubiquitin; Ubiquitin B

UniProt

P0CG47

Accession Number

P0CG47

Expression System

E.coli

Tag

N-His

Sequence

Lys 103-Cys 229

Field of Research

Cell biology; Neuroscience; epigenetics and nuclear signal

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.