Recombinant Human PAP protein (His tag)
Product Specifications
Background
Human PAP, also known as 28 kDa heat-and acid-stable phosphoprotein, PDGF-associated protein, PDGFA-associated protein 1, PDAP1, HASPP28, is a protein which belongs to the PDAP1 family. The encoded protein in rodents has been shown to bind PDGFA with a low affinity. PDGF-Associated Protein (PAP) is a phosphoprotein that may enhance PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. PDAP1 expression is induced by TNF-alpha, and cells overexpressing PDAP1 show significantly less apoptosis on exposure to TNF-alpha.
Abbreviation
PAP
Synonyms
HASPP28; PAP; PAP1; PDAP1; PDGFA-Associated Protein 1
UniProt
P08697
Accession Number
P08697
Expression System
E.coli
Tag
N-His
Sequence
Asn 40-Lys 491
Field of Research
Cell biology; Cardiovascular
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
49.6 kDa
Observed Molecular Weight
50 kDa
Species
Human
Available Sizes
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