Recombinant Human Rock-2 protein (His tag)

Product Specifications

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Abbreviation

Rock-2

Synonyms

ROCK 2; ROCK2; ROK alpha; ROKalpha; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; Rock II; Rock2; Rock2m

UniProt

O75116

Accession Number

O75116

Expression System

E.coli

Tag

N-His

Sequence

Leu 904-Gly 1140

Field of Research

Cancer; Cell Biology; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.