Recombinant Human PPAR-delta protein (His tag)

Product Specifications

Background

Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues.Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand

Abbreviation

PPAR-delta

Synonyms

NR1C2; NUC1; NUCI; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; PPAR-beta; PPAR-delta; PPARB; Peroxisome proliferator-activated receptor beta; Peroxisome proliferator-activated receptor delta

UniProt

Q03181

Accession Number

Q03181

Expression System

E.coli

Tag

N-His

Sequence

Leu260-Glu426

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.