Recombinant Human LDHA protein (His tag)

Product Specifications

Background

L-Lactate Dehydrogenase A Chain (LDHA) is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA contains an N-terminal coenzyme binding region, a central catalytic site, and at least nine utilized Lys acetylation and two Tyr phosphorylation sites. LDHA belongs to the lactate dehydrogenase family, expressed predominantly in muscle tissue. LDHA mutations have been linked to exertional myoglobinuria.

Abbreviation

LDHA

Synonyms

Cell Proliferation-Inducing Gene 19 Protein; L-Lactate Dehydrogenase A Chain; LDH Muscle Subunit; LDH-A; LDH-M; LDHA; Renal Carcinoma Antigen NY-REN-59

UniProt

P00338

Accession Number

P00338

Expression System

E.coli

Tag

N-His

Sequence

Ala 2-Phe 332

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.