Recombinant Human NEF3 protein (GST tag)

Product Specifications

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

Abbreviation

NEF3

Synonyms

160 kDa neurofilament protein; NEF3; NEFM; NF-M; NFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament triplet M protein

UniProt

P07197

Accession Number

P07197

Expression System

E.coli

Tag

N-GST

Sequence

Ser 2-Ser 358

Field of Research

Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.