Recombinant Human GSK3α Protein

Product Specifications

Background

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1.

Abbreviation

GSK3α

Synonyms

GSK-3 alpha; GSK3A; Glycogen synthase kinase-3 alpha; Serine/threonine-protein kinase GSK3A

UniProt

P49840

Accession Number

P49840

Expression System

E.coli

Tag

N-His

Sequence

Ser97-Pro448

Field of Research

Stem cells; Cardiovascular; Cell Biology; Neuroscience; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.