Recombinant Human HIF1A Protein
Product Specifications
Background
Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters.
Abbreviation
HIF1A
Synonyms
ARNT-interacting protein; BHLHE78; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; HIF-1-alpha; HIF1-alpha; Hypoxia-inducible factor 1-alpha; MOP1; Member of PAS protein 1; PAS domain-containing protein 8; PASD8; bHLHe78
UniProt
Q16665
Accession Number
Q16665
Expression System
E.coli
Tag
N-His
Sequence
Ser 576-Gln785
Field of Research
Immunology; Cardiovascular
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/9dwll9
Product MSDS
https://789.bio/eb/tL8daL
Calculated Molecular Weight
25.2 kDa
Observed Molecular Weight
26 kDa
Species
Human
Available Sizes
Curated Selection
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