Recombinant Annexin V-EGFP Protein (His Tag)

Product Specifications

Background

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.annexin A5, calcium and phospholipid binding protein, endonexin 2, placental protein 4, anchorin CII collagen receptor of chondrocytes, predominantly expressed in major constituent of growth plate chondrocytes, down-regulated in adult articular cartilage.EGFP can be used to detect apoptosis by flow cytometry or fluorescence microscopy.

Abbreviation

Annexin V

Synonyms

ANXA5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; Placental anticoagulant protein I; Thromboplastin inhibitor; Unconjugated Annexin V; Vascular anticoagulant-alpha

UniProt

P08758; C5MKY7

Accession Number

P08758&C5MKY7

Expression System

E.coli

Tag

N-His

Sequence

Annexin V (1Met-320Asp) - (GGGGS) 4-EGFP (1Met-239Lys)

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.