Recombinant Human CA199 (FUT3) Protein (His Tag)
Product Specifications
Background
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination, Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Abbreviation
CA199
Synonyms
Lewis FT; Blood group Lewis alpha-4-fucosyltransferase; FT3B; FUT3; FucT-III; Fucosyltransferase 3; Fucosyltransferase III; Galactoside 3 (4) -L-fucosyltransferase; LE
UniProt
P21217
Accession Number
P21217-1
Expression System
E.coli
Tag
N-His
Sequence
Val35-Thr361
Field of Research
Cardiovascular
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 80% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
38.1 kDa
Observed Molecular Weight
40.9 kDa
Species
Human
Available Sizes
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